![]() This makes it difficult, if not impossible, to detect important cellular structures and their distinguishing characteristics without artificially treating specimens. In their natural state, most of the cells and microorganisms that we observe under the microscope lack color and contrast. Explain the procedures and name clinical applications for Gram, endospore, acid-fast, negative capsule, and flagella staining.Describe the unique features of commonly used stains.Differentiate between simple and differential stains.J Clin Microbiol 26:1861–1863.By the end of this section, you will be able to: Simple method of inducing sporulation by Apophysomyces elegans and Saksenaea vasiformis. Improved methods for isolation and enumeration of Malassezia furfur from human skin. Comparison of inhibitory mold agar to Sabouraud dextrose agar as a primary medium for isolation of fungi. Scognamiglio T, Zinchuk R, Gumpeni P, Larone DH. Mycology Unit Women's and Children's Hospital, Adelaide, Australia. Ellis D, Davis S, Alexiou H, Handke R, Bartley R. Yeast identification-past, present, and future methods. CHROMagar Candida as the sole primary medium for isolation of yeasts and as a source medium for the rapid-assimilation-of-trehalose test. CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species. A new caffeic acid minimal synthetic medium for the rapid identification of Cryptococcus neoformans isolates. Vidotto V, Aoki S, Pontón J, Quindós G, Koga-Ito CY, Pugliese A. A comparison of modified methenamine silver and toluidine blue stains for the detection of Pneumocystis carinii in bronchoalveolar lavage specimens from immunosuppressed patients. Lacto-fuchsin: a new medium for mounting fungi. Evaluation of calcofluor white stain for detection of Pneumocystis carinii. ![]() Kim YK, Parulekar S, Yu PK, Pisani RJ, Smith TF, Anhalt JP. Use of calcofluor white in clinical mycology. Color atlas and text of the histopathology of mycotic diseases. Histopathologic diagnosis of fungal infections in the 21st century. National Committee for Clinical Laboratory Standards, Wayne, PA. Quality Control for Commercially Prepared Microbiological Culture Media. National Committee for Clinical Laboratory Standards. Development and validation of benomyl birdseed agar for the isolation of Cryptococcus neoformans and Cryptococcus gattii from environmental samples. Pham CD, Ahn S, Turner LA, Wohrle R, Lockhart SR. The benomyl test as a fundamental diagnostic method for medical mycology. Clinical and Laboratory Standards Institute, Wayne, PA. Principles and Procedures for Detection of Fungi in Clinical Specimens-Direct Examination and Culture. ![]() Clinical and Laboratory Standards Institute. Adelaide Medical Centre for Women and Children, Adelaide, Australia. Kidd SE, Halliday CL, Alexou H, Ellis DH. Wiley-Blackwell, West Sussex, United Kingdom. Identification of Pathogenic Fungi, 2nd ed. Identifying Fungi: A Clinical Laboratory Handbook, 2nd ed. Handbook of Media for Clinical Microbiology, 2nd ed. Department of Health and Human Services, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. World Health Organization, Geneva, Switzerland. Minister of Population and Public Health Branch Centre for Emergency Preparedness and Response, Ottawa, Canada. Best M, Graham ML, Leitner R, Ouellette M, Ugwu K. Oxford University Press, Inc, New York, NY.
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